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到哺乳动物的上皮细胞在体外细菌粘附的含量
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
In Vitro Assay of Bacterial Adhesion onto Mammalian Epithelial Cells
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到哺乳动物的上皮细胞在体外细菌粘附的含量

DOI:
10.3791/2783-v

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05:57 min

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May 16, 2011

•

, , , ,
1Universite de Montreal,Groupe de Recherche sur les Maladies Infectieuses du Porc GREMIP, Faculte de medecine veterinaire

Capítulos

  • 00:05Título
  • 00:58Prepare Bacterial Strains and Epithelial Cells
  • 02:34Infection of Cells
  • 04:15Representative cfu Counting Results
  • 05:35Conclusion

Summary

Tadução automática

Tadução automática

此协议是一个简单的细菌粘附实验，计数菌落形成单位，坚持到培养细胞的数量。该检测是可靠的，独立的黏附研究，并有众多的变化在大多数实验室中使用细菌发病机制的工作。

Tags

In Vitro AssayBacterial AdhesionMammalian Epithelial CellsBacterial PathogensAdhesinsHost Cell Surface ReceptorsCellular MicrobiologyBacterial Pathogenesis StudiesAdherence AssayEscherichia Coli Strain 27875Autotransporter Adhesin Involved In Diffuse Adherence (AIDA)AidA GeneHEp-2 Human Epithelial Cell Line

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