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In Vitro Assay of Bacterial Adhesion onto Mammalian Epithelial Cells
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
In Vitro Assay of Bacterial Adhesion onto Mammalian Epithelial Cells

In Vitro Assay of Bacterial Adhesion onto Mammalian Epithelial Cells

DOI:
10.3791/2783-v

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05:57 min

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May 16, 2011

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, , , ,
1Universite de Montreal,Groupe de Recherche sur les Maladies Infectieuses du Porc GREMIP, Faculte de medecine veterinaire

Capítulos

  • 00:05Título
  • 00:58Prepare Bacterial Strains and Epithelial Cells
  • 02:34Infection of Cells
  • 04:15Representative cfu Counting Results
  • 05:35Conclusion

Summary

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This protocol is a simple bacterial adhesion assay consisting in counting the numbers of bacterial colony forming units that are adhered onto cultured cells. The assay is robust, independent of the adhesin studied, and numerous variations are used in most laboratories working on bacterial pathogenesis.

Tags

In Vitro AssayBacterial AdhesionMammalian Epithelial CellsBacterial PathogensAdhesinsHost Cell Surface ReceptorsCellular MicrobiologyBacterial Pathogenesis StudiesAdherence AssayEscherichia Coli Strain 27875Autotransporter Adhesin Involved In Diffuse Adherence (AIDA)AidA GeneHEp-2 Human Epithelial Cell Line

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