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Non-surgical Intratracheal Instillation of Mice with Analysis of Lungs and Lung Draining Lymph Nodes by Flow Cytometry
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Non-surgical Intratracheal Instillation of Mice with Analysis of Lungs and Lung Draining Lymph Nodes by Flow Cytometry

Non-surgical Intratracheal Instillation of Mice with Analysis of Lungs and Lung Draining Lymph Nodes by Flow Cytometry

55,916 Views

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08:01 min

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May 02, 2011

DOI:

10.3791/2702-v

DOI:
10.3791/2702-v

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08:01 min
May 02, 2011

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8 Views
, , , , ,
1Department of Immunology,University of Colorado School of Medicine, 2Division of Cell Biology, Department of Pediatrics,National Jewish Health , 3Department of Microbiology, Immunology, and Pathology,Colorado State University, 4Department of Immunology,National Jewish Health

Tags

Non-surgicalIntratracheal InstillationMiceAnalysisLungsLung Draining Lymph NodesFlow CytometryPhagocytic CellsAlveolar MacrophagesLung Dendritic CellsAntigensImmune ResponseInhaled AntigensT CellsIntranasal AdministrationAerosolized ExposureTechnical SkillsLimitationsUpper Respiratory TractInterpretation Of ResultsSwallowingSneezingBreathing Rate Of The MouseDoses DeliveredI.t RouteTest MaterialsBlind Intubation

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Rayamajhi, M., Redente, E. F., Condon, T. V., Gonzalez-Juarrero, M., Riches, D. W., Lenz, L. L. Non-surgical Intratracheal Instillation of Mice with Analysis of Lungs and Lung Draining Lymph Nodes by Flow Cytometry. J. Vis. Exp. (51), e2702, doi:10.3791/2702 (2011).

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