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Non-surgical Intratracheal Instillation of Mice with Analysis of Lungs and Lung Draining Lymph Nodes by Flow Cytometry
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Non-surgical Intratracheal Instillation of Mice with Analysis of Lungs and Lung Draining Lymph Nodes by Flow Cytometry
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May 02, 2011
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We illustrate non-surgical delivery of test materials into the lungs of anesthetized mice via the trachea. This method permits lung exposure to bacterial and viral pathogens, cytokines, antibodies, beads, chemicals, or dyes. We further describe harvesting and processing of lungs and lung draining lymph nodes (LDLNs) for flow cytometry.
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Non-surgicalIntratracheal InstillationMiceAnalysisLungsLung Draining Lymph NodesFlow CytometryPhagocytic CellsAlveolar MacrophagesLung Dendritic CellsAntigensImmune ResponseInhaled AntigensT CellsIntranasal AdministrationAerosolized ExposureTechnical SkillsLimitationsUpper Respiratory TractInterpretation Of ResultsSwallowingSneezingBreathing Rate Of The MouseDoses DeliveredI.t RouteTest MaterialsBlind Intubation
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Rayamajhi, M., Redente, E. F., Condon, T. V., Gonzalez-Juarrero, M., Riches, D. W., Lenz, L. L. Non-surgical Intratracheal Instillation of Mice with Analysis of Lungs and Lung Draining Lymph Nodes by Flow Cytometry. J. Vis. Exp. (51), e2702, doi:10.3791/2702 (2011).
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