/
/
Dissection and Imaging of Active Zones in the Drosophila Neuromuscular Junction
É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Dissection and Imaging of Active Zones in the Drosophila Neuromuscular Junction
15,161 Views
•
06:05 min
•
April 27, 2011
DOI:
Summary
Automatically generated
The neuromuscular junction (NMJ) of Drosophila melanogaster is an important model system for studying normal synaptic function as well as perturbations to synaptic function found in certain neurological diseases. We present a protocol for dissection of the Drosophila larval motor system and immunostaining for active zone proteins within the NMJ.
Explore More Videos
DissectionImagingActive ZonesDrosophila Neuromuscular JunctionSynaptic StructureSynaptic FunctionGenetic ManipulationsLarval Nervous SystemNeurological DiseaseSynaptic MoleculesGlutamatergicActivity-dependent RemodelingIdentified Synaptic TerminalsMotor NeuronsBody-wall Muscle FibersEvolutionarily Conserved Synapse ComponentsMechanisms Underlying Synaptic FunctionMonoclonal AntibodyBruchpilot (Brp)CAST1/ERC Family Member
Read Article
Cite this Article
Smith, R., Taylor, J. P. Dissection and Imaging of Active Zones in the Drosophila Neuromuscular Junction. J. Vis. Exp. (50), e2676, doi:10.3791/2676 (2011).
Copy