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Dissection and Imaging of Active Zones in the Drosophila Neuromuscular Junction
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Dissection and Imaging of Active Zones in the Drosophila Neuromuscular Junction
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April 27, 2011
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The neuromuscular junction (NMJ) of Drosophila melanogaster is an important model system for studying normal synaptic function as well as perturbations to synaptic function found in certain neurological diseases. We present a protocol for dissection of the Drosophila larval motor system and immunostaining for active zone proteins within the NMJ.
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DissectionImagingActive ZonesDrosophila Neuromuscular JunctionSynaptic StructureSynaptic FunctionGenetic ManipulationsLarval Nervous SystemNeurological DiseaseSynaptic MoleculesGlutamatergicActivity-dependent RemodelingIdentified Synaptic TerminalsMotor NeuronsBody-wall Muscle FibersEvolutionarily Conserved Synapse ComponentsMechanisms Underlying Synaptic FunctionMonoclonal AntibodyBruchpilot (Brp)CAST1/ERC Family Member
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Smith, R., Taylor, J. P. Dissection and Imaging of Active Zones in the Drosophila Neuromuscular Junction. J. Vis. Exp. (50), e2676, doi:10.3791/2676 (2011).
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