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Whole Cell Aufnahme von einem organotypischen Vorbereitung der Neocortex
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10:29 min
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June 03, 2011
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Whole Cell RecordingOrganotypic Slice PreparationNeocortexVoltage-gated Potassium ChannelsPyramidal NeuronsGenetic ApproachOrganotypic Culture PreparationLaminar Pattern Of CortexAcute Neocortical SlicesVisually-identified Pyramidal NeuronsInfrared IlluminationDifferential Interference Contrast MicroscopyWhole Cell Patch ClampCurrent-clampVoltage-clampBiolistic TransfectionWild Type Potassium Channel DNAMutant Potassium Channel DNAFunction StudyEpifluorescence MicroscopyGreen Fluorescent Protein (GFP)Adjacent Untransfected Neurons
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Foehring, R. C., Guan, D., Toleman, T., Cantrell, A. R. Whole Cell Recording from an Organotypic Slice Preparation of Neocortex. J. Vis. Exp. (52), e2600, doi:10.3791/2600 (2011).
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