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Detection of Post-translational Modifications on Native Intact Nucleosomes by ELISA
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Biologia
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JoVE Journal Biologia
Detection of Post-translational Modifications on Native Intact Nucleosomes by ELISA

Detection of Post-translational Modifications on Native Intact Nucleosomes by ELISA

17,081 Views

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07:13 min

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April 26, 2011

DOI:

10.3791/2593-v

DOI:
10.3791/2593-v

•

07:13 min
April 26, 2011

•

8 Views
, , , ,
1Institute for Stem Cell Biology and Regenerative Medicine,Stanford University , 2Department of Molecular and Cell Biology,University of Connecticut, 3Department of Pharmaceutical Sciences,University of Connecticut

Summary

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Nucleosome ELISA (NU-ELISA) is a sensitive and quantitative method to detect global patterns of post-translational modifications in preparations of native, intact nucleosomes. These modifications include methylations, acetylations, and phosphorylations at specific histone amino acid residues, and hence NU-ELISA provides a global proteomic assay of the overall chromatin modification states of specific cell types.

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Cite this Article

Dai, B., Dahmani, F., Cichocki, J. A., Swanson, L. C., Rasmussen, T. P. Detection of Post-translational Modifications on Native Intact Nucleosomes by ELISA. J. Vis. Exp. (50), e2593, doi:10.3791/2593 (2011).

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