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Detection of Post-translational Modifications on Native Intact Nucleosomes by ELISA
JoVE Journal
生物学
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JoVE Journal 生物学
Detection of Post-translational Modifications on Native Intact Nucleosomes by ELISA

Detection of Post-translational Modifications on Native Intact Nucleosomes by ELISA

DOI:
10.3791/2593-v

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07:13 min

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April 26, 2011

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, , , ,
1Institute for Stem Cell Biology and Regenerative Medicine,Stanford University , 2Department of Molecular and Cell Biology,University of Connecticut, 3Department of Pharmaceutical Sciences,University of Connecticut

章

  • 00:05標題
  • 01:26Isolation of Nuclei
  • 03:07Isolation of Nucleosomes by in situ Micrococcal Nuclease Digestion
  • 04:31Nucleosome-ELISA (NU-ELISA)
  • 06:45Conclusion

概要

自動翻訳

自動翻訳

Nucleosome ELISA (NU-ELISA) is a sensitive and quantitative method to detect global patterns of post-translational modifications in preparations of native, intact nucleosomes. These modifications include methylations, acetylations, and phosphorylations at specific histone amino acid residues, and hence NU-ELISA provides a global proteomic assay of the overall chromatin modification states of specific cell types.

タグ

Post-translational ModificationsNative Intact NucleosomesELISAChromatinNucleosomeHistonesN-terminal TailsAcetylationMethylationPTMsEpigenetic Gene RegulationNucleosome ELISA (NU-ELISA)Global PTM SignaturesEpiproteomic State

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