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单克隆抗体的反对消极行政 H。 capsulatum和其他真菌病原体

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Imunologia e Infecção

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JoVE Journal Imunologia e Infecção

Passive Administration of Monoclonal Antibodies Against H. capsulatum and Others Fungal Pathogens

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单克隆抗体的反对消极行政 H。 capsulatum和其他真菌病原体

DOI:

•

09:57 min

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February 14, 2011

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Allan J. Guimarães, Luis R. Martinez, Joshua D. Nosanchuk

¹Department of Microbiology and Immunology,Albert Einstein College of Medicine

Capítulos

00:05Título
02:01Growth of H. capsulatum
03:50Hybridoma Growth and Purification of Monoclonal Antibodies
04:37Mouse Anesthesia and Intranasal Infections
06:22Survival Studies
07:17CFU Studies
08:27Monoclonal Antibody Protection Against H. capsulatum is Dependent on Antibody Specificity and Isotype
09:18Conclusion

Summary

Tadução automática

C57BL / 6小鼠已被用于研究HC发病机制，并提供最好的模式。我们正在探索这种真菌对体液免疫的潜在利益，并产生了几个单克隆抗体[组蛋白H2B和热休克蛋白60kDa]，我们为他们的腹腔给药后的保护效果测试。

Tags

Passive Administration Monoclonal Antibodies H. Capsulatum Fungal Pathogens Histoplasmosis Antibody Therapy Virulence Factors MAbs Generation Solid Phase ELISA System Splenocytes Fusion C57BL/6 Mice Infection Assessment Mortality As An Endpoint Colony Forming Units (CFU)Survival Studies Intervention Efficacy Vaccine Efficacy Virulence Testing

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