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An Analytical Tool-box for Comprehensive Biochemical, Structural and Transcriptome Evaluation of Oral Biofilms Mediated by Mutans Streptococci
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An Analytical Tool-box for Comprehensive Biochemical, Structural and Transcriptome Evaluation of Oral Biofilms Mediated by Mutans Streptococci
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January 25, 2011
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Biofilms formed on tooth surfaces are highly complex and exposed to constant innate and exogenous environmental challenges, which modulate their architecture, physiology and transcriptome. We developed a toolbox to examine the composition, structural organization and gene expression of oral biofilms, which can be adapted to other areas of biofilm research.
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Analytical Tool-boxComprehensive Biochemical EvaluationStructural EvaluationTranscriptome EvaluationOral BiofilmsMutans StreptococciMicrobial CellsExtracellular MatrixBiofilm DevelopmentCell ClustersMicrocoloniesExtracellular EnzymesSucrose SubstrateDental BiofilmsExopolysaccharides (EPS)Caries DiseaseGlucosyltransferasesTooth SurfacesEnvironmental ChallengesDiet-host-microbial InteractionsOral CavityMatrix CompositionPhysiology Profile
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Klein, M. I., Xiao, J., Heydorn, A., Koo, H. An Analytical Tool-box for Comprehensive Biochemical, Structural and Transcriptome Evaluation of Oral Biofilms Mediated by Mutans Streptococci. J. Vis. Exp. (47), e2512, doi:10.3791/2512 (2011).
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