/
/
An Analytical Tool-box for Comprehensive Biochemical, Structural and Transcriptome Evaluation of Oral Biofilms Mediated by Mutans Streptococci
È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
An Analytical Tool-box for Comprehensive Biochemical, Structural and Transcriptome Evaluation of Oral Biofilms Mediated by Mutans Streptococci
17,594 Views
•
11:09 min
•
January 25, 2011
DOI:
Summary
Automatically generated
Biofilms formed on tooth surfaces are highly complex and exposed to constant innate and exogenous environmental challenges, which modulate their architecture, physiology and transcriptome. We developed a toolbox to examine the composition, structural organization and gene expression of oral biofilms, which can be adapted to other areas of biofilm research.
Explore More Videos
Analytical Tool-boxComprehensive Biochemical EvaluationStructural EvaluationTranscriptome EvaluationOral BiofilmsMutans StreptococciMicrobial CellsExtracellular MatrixBiofilm DevelopmentCell ClustersMicrocoloniesExtracellular EnzymesSucrose SubstrateDental BiofilmsExopolysaccharides (EPS)Caries DiseaseGlucosyltransferasesTooth SurfacesEnvironmental ChallengesDiet-host-microbial InteractionsOral CavityMatrix CompositionPhysiology Profile
Read Article
Cite this Article
Klein, M. I., Xiao, J., Heydorn, A., Koo, H. An Analytical Tool-box for Comprehensive Biochemical, Structural and Transcriptome Evaluation of Oral Biofilms Mediated by Mutans Streptococci. J. Vis. Exp. (47), e2512, doi:10.3791/2512 (2011).
Copy