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Localizing Protein in 3D Neural Stem Cell Culture: a Hybrid Visualization Methodology
JoVE Journal
Neurociência
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JoVE Journal Neurociência
Localizing Protein in 3D Neural Stem Cell Culture: a Hybrid Visualization Methodology

Localizing Protein in 3D Neural Stem Cell Culture: a Hybrid Visualization Methodology

12,493 Views

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21:47 min

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December 19, 2010

DOI:

10.3791/2483-v

DOI:
10.3791/2483-v

•

21:47 min
December 19, 2010

•

1 Views
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1Neural Regeneration Laboratory and Ottawa Institute of Systems Biology, Department of Biochemistry, Microbiology and Immunology,University of Ottawa, 2Carleton Immersive Media Studio, Azrieli School of Architecture and Urbanism,Carleton University

Summary

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Here, we describe how to produce, expand, and immunolabel postnatal hippocampal neural progenitor cells (NPCs) in three-dimensional (3D) culture. Next, using hybrid visualization technologies, we demonstrate how digital images of immunolabelled cryosections can be used to reconstruct and map the spatial position of immunopositive cells throughout the entire 3D neurosphere.

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Imbeault, S., Valenzuela, N., Fai, S., Bennett, S. A. Localizing Protein in 3D Neural Stem Cell Culture: a Hybrid Visualization Methodology. J. Vis. Exp. (46), e2483, doi:10.3791/2483 (2010).

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