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Localizing Protein in 3D Neural Stem Cell Culture: a Hybrid Visualization Methodology
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神経科学
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JoVE Journal 神経科学
Localizing Protein in 3D Neural Stem Cell Culture: a Hybrid Visualization Methodology

Localizing Protein in 3D Neural Stem Cell Culture: a Hybrid Visualization Methodology

DOI:
10.3791/2483-v

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21:47 min

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December 19, 2010

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, , ,
1Neural Regeneration Laboratory and Ottawa Institute of Systems Biology, Department of Biochemistry, Microbiology and Immunology,University of Ottawa, 2Carleton Immersive Media Studio, Azrieli School of Architecture and Urbanism,Carleton University

章

  • 00:01標題
  • 00:56Introduction
  • 01:37Isolation of Neural Progenitor Cells
  • 05:50Serial Cryosectioning
  • 07:03Immunocytochemistry
  • 08:49Imaging
  • 09:17Alignment
  • 10:23Cell Typology
  • 12:30Cell Mapping
  • 13:48Importing and Assembling Maps in 3-D
  • 15:25Locating Progenitor Cell Typologies in 3-D
  • 19:23Rendering/Compositing
  • 21:05Conclusion

概要

自動翻訳

自動翻訳

Here, we describe how to produce, expand, and immunolabel postnatal hippocampal neural progenitor cells (NPCs) in three-dimensional (3D) culture. Next, using hybrid visualization technologies, we demonstrate how digital images of immunolabelled cryosections can be used to reconstruct and map the spatial position of immunopositive cells throughout the entire 3D neurosphere.

タグ

Protein Localization3D Neural Stem Cell CultureVisualization MethodologyNeurospheresDevelopmental LineagesImmunocytochemistryTarget ProteinsNPC FateCellular PositioningCryosectionsConfocal Reconstructions

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