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ニューロスフェアのアッセイを使用してマウス胎児神経幹細胞培養の確立
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Neurociência
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JoVE Journal Neurociência
Establishing Embryonic Mouse Neural Stem Cell Culture Using the Neurosphere Assay
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ニューロスフェアのアッセイを使用してマウス胎児神経幹細胞培養の確立

24,327 Views

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11:54 min

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January 11, 2011

DOI:

10.3791/2457-v

DOI:
10.3791/2457-v

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11:54 min
January 11, 2011

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2 Views
, , , ,
1Department of Anatomical Sciences,Shiraz University of Medical Sciences, Shiraz, Iran , 2Department of Neurosurgery,The University of Florida

Tags

Embryonic Mouse Neural Stem Cell CultureNeurosphere AssayStem CellsUndifferentiated CellsCell GenesisAging ProcessInjury And DiseaseNeural Stem Cells (NSCs)Serum-free Culture SystemIsolation And Expansion Of NSCsEmbryonic CNSIn Vitro Expanded NSCsCNS Cell TypesReliable And Consistent ResultsE14 Mouse EmbryosBrain MicrodissectionGanglionic EminencesSingle Cell SuspensionNSA CulturePrimary Neurospheres

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Azari, H., Sharififar, S., Rahman, M., Ansari, S., Reynolds, B. A. Establishing Embryonic Mouse Neural Stem Cell Culture Using the Neurosphere Assay. J. Vis. Exp. (47), e2457, doi:10.3791/2457 (2011).

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