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建立小鼠胚胎神经干细胞培养神经球含量
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JoVE Journal Neurociência
Establishing Embryonic Mouse Neural Stem Cell Culture Using the Neurosphere Assay
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建立小鼠胚胎神经干细胞培养神经球含量

24,327 Views

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11:54 min

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January 11, 2011

DOI:

10.3791/2457-v

DOI:
10.3791/2457-v

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11:54 min
January 11, 2011

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2 Views
, , , ,
1Department of Anatomical Sciences,Shiraz University of Medical Sciences, Shiraz, Iran , 2Department of Neurosurgery,The University of Florida

Tags

Embryonic Mouse Neural Stem Cell CultureNeurosphere AssayStem CellsUndifferentiated CellsCell GenesisAging ProcessInjury And DiseaseNeural Stem Cells (NSCs)Serum-free Culture SystemIsolation And Expansion Of NSCsEmbryonic CNSIn Vitro Expanded NSCsCNS Cell TypesReliable And Consistent ResultsE14 Mouse EmbryosBrain MicrodissectionGanglionic EminencesSingle Cell SuspensionNSA CulturePrimary Neurospheres

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Azari, H., Sharififar, S., Rahman, M., Ansari, S., Reynolds, B. A. Establishing Embryonic Mouse Neural Stem Cell Culture Using the Neurosphere Assay. J. Vis. Exp. (47), e2457, doi:10.3791/2457 (2011).

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