É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
ニューロスフェアのアッセイを使用してマウス胎児神経幹細胞培養の確立
24,365 Views
•
11:54 min
•
January 11, 2011
DOI:
Summary
Automatically generated
このビデオプロトコルは、胚14日目マウスの脳の神経節隆起から神経幹細胞の単離と拡大のためのニューロスアッセイのアプリケーションを示しています。
Explore More Videos
Embryonic Mouse Neural Stem Cell CultureNeurosphere AssayStem CellsUndifferentiated CellsCell GenesisAging ProcessInjury And DiseaseNeural Stem Cells (NSCs)Serum-free Culture SystemIsolation And Expansion Of NSCsEmbryonic CNSIn Vitro Expanded NSCsCNS Cell TypesReliable And Consistent ResultsE14 Mouse EmbryosBrain MicrodissectionGanglionic EminencesSingle Cell SuspensionNSA CulturePrimary Neurospheres
Read Article
Cite this Article
Azari, H., Sharififar, S., Rahman, M., Ansari, S., Reynolds, B. A. Establishing Embryonic Mouse Neural Stem Cell Culture Using the Neurosphere Assay. J. Vis. Exp. (47), e2457, doi:10.3791/2457 (2011).
Copy