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Capítulos
- 00:05Título
- 01:23Introduction
- 01:49Setting Up
- 02:33Fixing Flies in Collars
- 03:12Paraffin Sections of Drosophila Thoraxes
- 04:38Cryosections of Drosophila Thoraxes
- 05:39Lipid Detection in Drosophila Muscles
- 06:18Thoracic Muscles of Drosophila
- 07:08Conclusion
Identificação dos mecanismos subjacentes à lesão muscular é crucial. Aqui apresentamos a técnica histológica para preparar parafina e congeladas seções do Drosophila músculos do tórax. Isto permite a análise da morfologia do músculo e localização de proteínas e outros componentes celulares do músculo.
Tags
Paraffin-embedded SectionsFrozen SectionsDrosophila Adult MusclesMolecular CharacterizationMuscular DystrophiesMyopathiesMuscle DiseaseGenetic AnalysisMuscle SpecificationMuscle FormationMuscle FunctionModel SystemsMuscle PhysiologyMuscle DamageDrosophila Multi-fiber MusclesVertebrate Striated MusclesDystrophic Muscle MorphologyMuscular FunctionAgeing Adult FliesHistological TechniqueStainingProtein Detecting DyesCryosectionsImmunohistochemical DetectionMorphological DefectsExpression PatternMuscle/neuron-specific Proteins
