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December 27, 2010
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Identification of mechanisms underlying muscle damage is crucial. Here we present the histological technique for preparing paraffin-embedded and frozen sections of Drosophila thoracic muscles. This allows analysis of muscle morphology and localization of protein and other muscle cell components.
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Paraffin-embedded SectionsFrozen SectionsDrosophila Adult MusclesMolecular CharacterizationMuscular DystrophiesMyopathiesMuscle DiseaseGenetic AnalysisMuscle SpecificationMuscle FormationMuscle FunctionModel SystemsMuscle PhysiologyMuscle DamageDrosophila Multi-fiber MusclesVertebrate Striated MusclesDystrophic Muscle MorphologyMuscular FunctionAgeing Adult FliesHistological TechniqueStainingProtein Detecting DyesCryosectionsImmunohistochemical DetectionMorphological DefectsExpression PatternMuscle/neuron-specific Proteins
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Kucherenko, M. M., Marrone, A. K., Rishko, V. M., Yatsenko, A. S., Klepzig, A., Shcherbata, H. R. Paraffin-Embedded and Frozen Sections of Drosophila Adult Muscles. J. Vis. Exp. (46), e2438, doi:10.3791/2438 (2010).
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