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初级运动和感觉神经元上的静电聚- L -乳酸纳米纤维支架中定义的媒体文化

JoVE Journal
Neurociência

This content is Free Access.

JoVE Journal Neurociência

The Culture of Primary Motor and Sensory Neurons in Defined Media on Electrospun Poly-L-lactide Nanofiber Scaffolds

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初级运动和感觉神经元上的静电聚- L -乳酸纳米纤维支架中定义的媒体文化

DOI:

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16:03 min

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February 15, 2011

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Michelle K. Leach, Zhang-Qi Feng, Caitlyn C. Gertz, Samuel J. Tuck, Tara M. Regan, Youssef Naim, Andrea M. Vincent, Joseph M. Corey^3,4

¹Department of Biomedical Engineering,University of Michigan, ²State Key Laboratory of Bioelectronics,Southeast University, ³Department of Neurology,University of Michigan , ⁴Geriatric Research, Education and Clinical Center,Veterans Affairs Ann Arbor Health System

Capítulos

00:05Título
01:43Preparation of Electrospun Fiber Substrates
04:40Primary Cell Culture of Rat Embryonic Neuron
06:53Motor Neuron (MN) Processing
09:29Sensory Neuron (SN) Processing
10:56Plating and Culture
12:41Representative Results
14:40Conclusion

Summary

Tadução automática

不结盟纤维直接的神经元在体外的生长，是一个潜在的神经再生的支架组成部分。我们描述了一个准备静电纤维基材和主大鼠E15感觉（DRG）和运动神经元无血清培养的过程。用免疫细胞化学神经元的可视化也包括在内。

Tags

Culture Primary Motor Neurons Sensory Neurons Defined Media Electrospun Nanofiber Scaffolds Electrospinning Technique Alignment Random Fibers Biodegradable Polymers Poly-L-lactic Acid (PLLA)Nerve Regeneration Primary Cell Culture System Biomaterials Dorsal Root Ganglia Explants Dissociated Neurons

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