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Imagerie en temps réel des leucotriènes B 4 Cell Mediated et BLT1 Interactions avec β-arrestine
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Capítulos
- 00:05Título
- 01:13Introduction
- 01:48Preparation of Bone Marrow Derived Dendritic Cells (BMDC)
- 02:42Preparation of Lipid Loaded Micropipette
- 04:20Live-cell Imaging of Cell Migration in Response to Ligand Application
- 05:25Representative Results for Migration of BMDCs
- 06:03Live Cell Imaging of Transfected RBL-2H3 Cells
- 09:49Image Analysis
- 10:51Representative Results
- 13:03Conclusion
Ce document décrit la méthodologie pour déterminer la réponse chimiotactique des leucocytes à des ligands spécifiques et d'identifier les interactions entre les récepteurs de surface cellulaire et des protéines cytosoliques en utilisant des techniques d'imagerie cellulaire direct.
Tags
Leukotriene B4Cell MigrationBLT1β-arrestinG-protein Coupled ReceptorsTransmembrane Protein FamilyExtracellular SignalsIntracellular ResponsesChemotaxisCalcium ReleaseGene RegulationLigand BindingConformational ChangesHeterotrimeric G-proteinsSecond MessengersCyclic Adenosine Monophosphate (cAMP)Inositol Triphosphate (IP3)Diacyl Glycerol (DG)DesensitizationSequestrationInternalizationG-protein Receptor Kinases (GRKs)Cytosolic ProteinsMembrane TranslocationPro-inflammatory Lipid MoleculeArachidonic Acid Pathway
