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白三烯B实时成像 4介导的细胞迁移和BLT1与β- arrestin的相互作用
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13:45 min
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December 23, 2010
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本文介绍的方法，以确定具体的配体的白细胞趋化反应和识别细胞表面的受体，并用活细胞成像技术的胞质蛋白质之间的相互作用。
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Leukotriene B4Cell MigrationBLT1β-arrestinG-protein Coupled ReceptorsTransmembrane Protein FamilyExtracellular SignalsIntracellular ResponsesChemotaxisCalcium ReleaseGene RegulationLigand BindingConformational ChangesHeterotrimeric G-proteinsSecond MessengersCyclic Adenosine Monophosphate (cAMP)Inositol Triphosphate (IP3)Diacyl Glycerol (DG)DesensitizationSequestrationInternalizationG-protein Receptor Kinases (GRKs)Cytosolic ProteinsMembrane TranslocationPro-inflammatory Lipid MoleculeArachidonic Acid Pathway
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Jala, V. R., Haribabu, B. Real-time Imaging of Leukotriene B4 Mediated Cell Migration and BLT1 Interactions with β-arrestin. J. Vis. Exp. (46), e2315, doi:10.3791/2315 (2010).
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