/
/
Real-time Imaging of Leukotriene B4 Mediated Cell Migration and BLT1 Interactions with β-arrestin
Se requiere una suscripción a JoVE para ver este contenido. Inicie sesión o comience su prueba gratuita.
Real-time Imaging of Leukotriene B4 Mediated Cell Migration and BLT1 Interactions with β-arrestin
12,688 Views
•
13:45 min
•
December 23, 2010
DOI:
Summary
Automatically generated
This paper describes the methodology to determine the chemotactic response of leukocytes to specific ligands and identify interactions between the cell surface receptors and cytosolic proteins using live cell imaging techniques.
Explore More Videos
Leukotriene B4Cell MigrationBLT1β-arrestinG-protein Coupled ReceptorsTransmembrane Protein FamilyExtracellular SignalsIntracellular ResponsesChemotaxisCalcium ReleaseGene RegulationLigand BindingConformational ChangesHeterotrimeric G-proteinsSecond MessengersCyclic Adenosine Monophosphate (cAMP)Inositol Triphosphate (IP3)Diacyl Glycerol (DG)DesensitizationSequestrationInternalizationG-protein Receptor Kinases (GRKs)Cytosolic ProteinsMembrane TranslocationPro-inflammatory Lipid MoleculeArachidonic Acid Pathway
Read Article
Cite this Article
Jala, V. R., Haribabu, B. Real-time Imaging of Leukotriene B4 Mediated Cell Migration and BLT1 Interactions with β-arrestin. J. Vis. Exp. (46), e2315, doi:10.3791/2315 (2010).
Copy