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In vivo Imaging of Intact Drosophila Larvae at Sub-cellular Resolution
Capítulos
- 00:00Título
- 00:05Introduction
- 02:01Preparing Larva
- 03:00Assembly of the Imaging Chamber
- 04:36Anesthetization of the Larva
- 07:48Imaging
- 14:39Recovery from Anesthetization
This protocol describes a reliable method for anesthetization and imaging of intact Drosophila melanogaster larvae. We have utilized the volatile anesthetic desflurane to allow for repetitive imaging at sub-cellular resolution and re-identification of structures for up to a few days1.
Tags
In Vivo ImagingDrosophila LarvaeSub-cellular ResolutionOptical ImagingGenetically Encoded FluorophoresGenetic ToolsTransgenic Animal LinesAnesthetize LarvaeDesfluraneSynaptic PopulationsHigh Degree Of AnesthetizationSurvival RateTime-pointsProteins DynamicsGlutamate Receptor Subunit GluR-IIAEndogenous PromoterExon Trap Line FasII-GFP
