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In vivo Imaging of Intact Drosophila Larvae at Sub-cellular Resolution
JoVE 杂志
生物学
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JoVE 杂志 生物学
In vivo Imaging of Intact Drosophila Larvae at Sub-cellular Resolution

In vivo Imaging of Intact Drosophila Larvae at Sub-cellular Resolution

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17:51 min

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September 10, 2010

DOI:

10.3791/2249-v

DOI:
10.3791/2249-v

•

17:51 min
September 10, 2010

•

1 Views
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1Junior Research Group Synaptic Plasticity, Hertie Institute for Clinical Brain Research,University of Tübingen, 2Graduate School of Cellular and Molecular Neuroscience,University of Tübingen

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This protocol describes a reliable method for anesthetization and imaging of intact Drosophila melanogaster larvae. We have utilized the volatile anesthetic desflurane to allow for repetitive imaging at sub-cellular resolution and re-identification of structures for up to a few days1.

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Zhang, Y., Füger, P., Hannan, S. B., Kern, J. V., Lasky, B., Rasse, T. M. In vivo Imaging of Intact Drosophila Larvae at Sub-cellular Resolution. J. Vis. Exp. (43), e2249, doi:10.3791/2249 (2010).

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