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Gene Delivery to Postnatal Rat Brain by Non-ventricular Plasmid Injection and Electroporation
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JoVE Journal Neurociência
Gene Delivery to Postnatal Rat Brain by Non-ventricular Plasmid Injection and Electroporation

Gene Delivery to Postnatal Rat Brain by Non-ventricular Plasmid Injection and Electroporation

14,890 Views

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10:34 min

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September 17, 2010

DOI:

10.3791/2244-v

DOI:
10.3791/2244-v

•

10:34 min
September 17, 2010

•

4 Views
, , ,
1Neuroscience Center,University of Helsinki, 2Faculty of Biological and Enviromental Sciences,University of Helsinki

Tags

Gene DeliveryPostnatal Rat BrainNon-ventricularPlasmid InjectionElectroporationTransgenic AnimalsGene Of InterestKnockout AnimalsCompensatory MechanismsGene Expression TimingTransfected CellsMicroinjectionIn Vivo ElectroporationTargeted DeliverySiRNADye MoleculesNewborn Rodent BrainNon-migratory Cell TypesTwo-photon In Vivo ImagingElectrophysiological Experiments

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Molotkov, D. A., Yukin, A. Y., Afzalov, R. A., Khiroug, L. S. Gene Delivery to Postnatal Rat Brain by Non-ventricular Plasmid Injection and Electroporation. J. Vis. Exp. (43), e2244, doi:10.3791/2244 (2010).

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