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Gene Delivery to Postnatal Rat Brain by Non-ventricular Plasmid Injection and Electroporation
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September 17, 2010
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概要
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This protocol describes a non-viral method of delivery of genetic constructs to a certain area of living rodent brain. The method consists of plasmid preparation, micropipette fabrication, neonatal rat pup surgery, microinjection of the construct, and in vivo electroporation.
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Gene DeliveryPostnatal Rat BrainNon-ventricularPlasmid InjectionElectroporationTransgenic AnimalsGene Of InterestKnockout AnimalsCompensatory MechanismsGene Expression TimingTransfected CellsMicroinjectionIn Vivo ElectroporationTargeted DeliverySiRNADye MoleculesNewborn Rodent BrainNon-migratory Cell TypesTwo-photon In Vivo ImagingElectrophysiological Experiments
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Molotkov, D. A., Yukin, A. Y., Afzalov, R. A., Khiroug, L. S. Gene Delivery to Postnatal Rat Brain by Non-ventricular Plasmid Injection and Electroporation. J. Vis. Exp. (43), e2244, doi:10.3791/2244 (2010).
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