/
/
Gene Delivery to Postnatal Rat Brain by Non-ventricular Plasmid Injection and Electroporation
Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
Gene Delivery to Postnatal Rat Brain by Non-ventricular Plasmid Injection and Electroporation
14,930 Views
•
10:34 min
•
September 17, 2010
DOI:
Summary
Automatically generated
This protocol describes a non-viral method of delivery of genetic constructs to a certain area of living rodent brain. The method consists of plasmid preparation, micropipette fabrication, neonatal rat pup surgery, microinjection of the construct, and in vivo electroporation.
Explore More Videos
Gene DeliveryPostnatal Rat BrainNon-ventricularPlasmid InjectionElectroporationTransgenic AnimalsGene Of InterestKnockout AnimalsCompensatory MechanismsGene Expression TimingTransfected CellsMicroinjectionIn Vivo ElectroporationTargeted DeliverySiRNADye MoleculesNewborn Rodent BrainNon-migratory Cell TypesTwo-photon In Vivo ImagingElectrophysiological Experiments
Read Article
Cite this Article
Molotkov, D. A., Yukin, A. Y., Afzalov, R. A., Khiroug, L. S. Gene Delivery to Postnatal Rat Brain by Non-ventricular Plasmid Injection and Electroporation. J. Vis. Exp. (43), e2244, doi:10.3791/2244 (2010).
Copy