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Blue Native Polyacrylamide Gel Electrophoresis (BN-PAGE) for Analysis of Multiprotein Complexes from Cellular Lysates

JoVE Journal
Biologia

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JoVE Journal Biologia

Blue Native Polyacrylamide Gel Electrophoresis (BN-PAGE) for Analysis of Multiprotein Complexes from Cellular Lysates

Blue Native Polyacrylamide Gel Electrophoresis (BN-PAGE) for Analysis of Multiprotein Complexes from Cellular Lysates

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12:03 min

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February 24, 2011

DOI:

DOI:

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12:03 min

February 24, 2011

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Gina J. Fiala^2,3, Wolfgang W. A. Schamel*^2,3, Britta Blumenthal*³

¹Spemann Graduate School of Biology and Medicine (SGBM),University of Freiburg, ²Centre for Biological Signalling Studies (bioss) and Biology III, Faculty of Biology,University of Freiburg, ³Department of Molecular Immunology,Max-Planck-Institute of Immunology and Epigenetics

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Blue Native Polyacrylamide Gel Electrophoresis BN-PAGE Multiprotein Complexes Cell Signalling Protein-protein Interaction Networks Native Conditions Resolution Gel Filtration Sucrose Density Ultracentrifugation MPC Size Composition Relative Abundance Hydrodynamic Size And Shape Polyacrylamide Matrix Cellular Lysates Lysate Dialysis First Dimension BN-PAGE Second Dimension SDS-PAGE

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