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Blue Native Polyacrylamide Gel Electrophoresis (BN-PAGE) for Analysis of Multiprotein Complexes from Cellular Lysates
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Blue Native Polyacrylamide Gel Electrophoresis (BN-PAGE) for Analysis of Multiprotein Complexes from Cellular Lysates

Blue Native Polyacrylamide Gel Electrophoresis (BN-PAGE) for Analysis of Multiprotein Complexes from Cellular Lysates

129,198 Views

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12:03 min

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February 24, 2011

DOI:

10.3791/2164-v

DOI:
10.3791/2164-v

•

12:03 min
February 24, 2011

•

66 Views
, ,
1Spemann Graduate School of Biology and Medicine (SGBM),University of Freiburg, 2Centre for Biological Signalling Studies (bioss) and Biology III, Faculty of Biology,University of Freiburg, 3Department of Molecular Immunology,Max-Planck-Institute of Immunology and Epigenetics

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In this video, we describe the characterization of multiprotein complexes (MPCs) by blue native polyacrylamide gel electrophoresis (BN-PAGE). In a first dimension, dialyzed cellular lysates are separated by BN-PAGE to identify individual MPCs. In a second dimension SDS-PAGE, MPCs of interest are further subdivided to analyze their constituents by immunoblotting.

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Fiala, G. J., Schamel, W. W. A., Blumenthal, B. Blue Native Polyacrylamide Gel Electrophoresis (BN-PAGE) for Analysis of Multiprotein Complexes from Cellular Lysates. J. Vis. Exp. (48), e2164, doi:10.3791/2164 (2011).

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