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在果蝇幼虫分部轴突在体内的突触小泡的可视化
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Neurociência
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JoVE Journal Neurociência
In vivo Visualization of Synaptic Vesicles Within Drosophila Larval Segmental Axons
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在果蝇幼虫分部轴突在体内的突触小泡的可视化

DOI:
10.3791/2151-v

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05:58 min

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October 15, 2010

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,
1Department of Biological Sciences,SUNY-University at Buffalo

Capítulos

  • 00:00Título
  • 00:49Introduction
  • 01:01Preparation for Dissection
  • 01:51Collection of Larvae
  • 02:16Live Dissection of Larvae
  • 04:18Live Imaging of Dissected Larvae
  • 05:00Representative Images
  • 05:37Conclusion

Summary

Tadução automática

Tadução automática

这个协议，讨论了现场清扫<em>果蝇</em>幼虫为目的的绿色荧光标记的微管轨道上轴突囊泡的运动成像。

Tags

In Vivo VisualizationSynaptic VesiclesDrosophila Larval Segmental AxonsAxonal TransportNeurological DiseasesDissection ProtocolLive ImagingUAS-GAL4 MethodGFP-tagged APPSynaptotagmin VesiclesNeuronal GAL4 DriversFluorescently Tagged MarkersMitochrondriaMitoTrackerLysosomesLysoTrackerGFP-vesicle MovementParticle Movement

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