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In vivo Visualization of Synaptic Vesicles Within Drosophila Larval Segmental Axons
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In vivo Visualization of Synaptic Vesicles Within Drosophila Larval Segmental Axons
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October 15, 2010
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This protocol discusses the live dissection of Drosophila larvae for the purpose of imaging the movement of GFP tagged axonal vesicles on microtubule tracks.
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In Vivo VisualizationSynaptic VesiclesDrosophila Larval Segmental AxonsAxonal TransportNeurological DiseasesDissection ProtocolLive ImagingUAS-GAL4 MethodGFP-tagged APPSynaptotagmin VesiclesNeuronal GAL4 DriversFluorescently Tagged MarkersMitochrondriaMitoTrackerLysosomesLysoTrackerGFP-vesicle MovementParticle Movement
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Kuznicki, M. L., Gunawardena, S. In vivo Visualization of Synaptic Vesicles Within Drosophila Larval Segmental Axons. J. Vis. Exp. (44), e2151, doi:10.3791/2151 (2010).
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