Journal
/
Neurociência
/
双荧光原位杂交食脑切片
JoVE Journal
Neurociência
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Neurociência
Double Fluorescence in situ Hybridization in Fresh Brain Sections
Please note that all translations are automatically generated. Click here for the English version.

双荧光原位杂交食脑切片

DOI:
10.3791/2102-v

•

12:15 min

•

August 14, 2010

•

, , ,
1Department of Brain and Cognitive Sciences,University of Rochester, 2Center for Visual Science,University of Rochester

Capítulos

  • 00:04Título
  • 00:07Introduction
  • 00:56Prep of Sephadex G-50 Columns
  • 02:54Labeling and Purification of Riboprobes
  • 04:25Post-fixation, Acetylation, and Hybridization
  • 07:22Detection and Visualization of Riboprobes
  • 10:35Representative Results
  • 11:35Conclusion

Summary

Tadução automática

Tadução automática

该协议涉及非放射性<em>原位</em>杂交程序，使在脊椎动物的大脑薄片，同时识别两个种成绩单，在一个单细胞的决议。

Tags

Double Fluorescence In Situ HybridizationDFISHMRNAFresh Frozen Brain SectionsGene Co-regulationAnatomical OrganizationNeurochemical PropertiesSensory ExperienceCentral Sensory CircuitsSingle Cell ResolutionProtocolValidationMiceRatsSongbirdsVertebrate Species

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

结合亲脂性染料，原位杂交，免疫组织化学和组织学

视网膜节的形态分析

设计和组装的超轻型电动微型硬盘（Microdrive）慢性神经录音的小动物

完全性脊髓损伤和脑解剖协议后续Wholemount<em>原位</em>杂交苗种海七鳃鳗

在脑切片检测轴突本地化mRNAs的高空间分辨率<em>原位</em>杂交

快速<em>原位</em使用寡核苷酸探针对大鼠多聚甲醛为前缀的大脑5-羟色胺综合征>杂交

结合双荧光<em>原位</em>杂交免疫标记为三个基因在小鼠大脑切片中表达的检测

通过RNA定位蝗虫天线中的气味受体基因<em>原位</em>杂交

新鲜人脑组织中毛细血管的分离

双直接注射血液到西斯特纳麦格作为苏巴拉奇内德出血的模型

Read Article