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Double Fluorescence in situ Hybridization in Fresh Brain Sections
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Journal JoVE Neurosciences
Double Fluorescence in situ Hybridization in Fresh Brain Sections

Double Fluorescence in situ Hybridization in Fresh Brain Sections

15,822 Views

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12:15 min

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August 14, 2010

DOI:

10.3791/2102-v

DOI:
10.3791/2102-v

•

12:15 min
August 14, 2010

•

4 Views
, , ,
1Department of Brain and Cognitive Sciences,University of Rochester, 2Center for Visual Science,University of Rochester

Summary

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This protocol involves a non-radioactive in-situ hybridization procedure that enables the simultaneous identification of two transcript species, at a single cell resolution, in thin sections of the vertebrate brain.

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Cite this Article

Jeong, J. K., Chen, Z., Tremere, L. A., Pinaud, R. Double Fluorescence in situ Hybridization in Fresh Brain Sections. J. Vis. Exp. (42), e2102, doi:10.3791/2102 (2010).

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