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质粒DNA重组流感病毒的一代

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Imunologia e Infecção

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JoVE Journal Imunologia e Infecção

Generation of Recombinant Influenza Virus from Plasmid DNA

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质粒DNA重组流感病毒的一代

DOI:

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11:31 min

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August 03, 2010

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Luis Martínez-Sobrido, Adolfo García-Sastre

¹Department of Microbiology and Immunology,University of Rochester School of Medicine and Dentistry, ²Departments of Microbiology and Medicine, and Global Health and Emerging Pathogens Institute,Mount Sinai School of Medicine

Capítulos

00:04Título
01:00Introduction
01:29Influenza Virus Rescue Transfection
04:54Infecting MDCK Cells & TC Harvest
06:14Egg Infection & Allonotic Fluid Harvest
07:55HA Assay
08:55Confirmation of Rescue
10:19Conclusion

Summary

Tadução automática

质粒DNA的A型流感病毒的救援是一个基本的和必要的实验技术，使流感的研究人员，以产生重组病毒在流感病毒的生物学特性研究的多个方面，被视为潜在的载体或疫苗使用。

Tags

Recombinant Influenza Virus Plasmid DNA Reverse Genetics Influenza Research Infectious Viruses Viral Proteins Mutations Viral Replication Pathogenesis Viral Vectors Foreign Proteins

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