Journal
/
Bioquímica
/
High-Throughput Quantification of the Synthesis and Distribution of mtDNA
/
Addition of Primary and Secondary Antibodies to the Transfected HeLa Cells to Monitor the mtDNA Distribution
JoVE Journal
Bioquímica
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Bioquímica
Addition of Primary and Secondary Antibodies to the Transfected HeLa Cells to Monitor the mtDNA Distribution

Addition of Primary and Secondary Antibodies to the Transfected HeLa Cells to Monitor the mtDNA Distribution

94 Views

•

02:04 min

•

May 05, 2023

DOI:

10.3791/200043-v

DOI:
10.3791/200043-v

•

02:04 min
May 05, 2023

•

1 Views
, , ,
1Faculty of Biology, Institute of Genetics and Biotechnology,University of Warsaw, 2Institute of Biochemistry and Biophysics,Polish Academy of Sciences, 3International Institute of Molecular and Cell Biology in Warsaw

Explore More Videos

Primary AntibodySecondary AntibodyTransfected HeLa CellsMitochondrial DNA DistributionBSAPBSAlexa Fluor 488Alexa Fluor 555Anti-DNA AntibodiesMitochondrial Transcription Factor A (TFAM)Fluorescence Quantification

Read Article

Cite this Article

Borowski, L. S., Kasztelan, K., Czerwinska-Kostrzewska, J., Szczesny, R. J. Addition of Primary and Secondary Antibodies to the Transfected HeLa Cells to Monitor the mtDNA Distribution. J. Vis. Exp. (Pending Publication), e200043, doi:10.3791/200043 (2023).

Download .ris file

Copy

Share Video

.

Copy