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High-Throughput Quantification of the Synthesis and Distribution of mtDNA
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Addition of Primary and Secondary Antibodies to the Transfected HeLa Cells to Monitor the mtDNA Distribution
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Addition of Primary and Secondary Antibodies to the Transfected HeLa Cells to Monitor the mtDNA Distribution

Addition of Primary and Secondary Antibodies to the Transfected HeLa Cells to Monitor the mtDNA Distribution

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02:04 min

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May 05, 2023

DOI:

10.3791/200043-v

DOI:
10.3791/200043-v

•

02:04 min
May 05, 2023

•

1 Views
, , ,
1Faculty of Biology, Institute of Genetics and Biotechnology,University of Warsaw, 2Institute of Biochemistry and Biophysics,Polish Academy of Sciences, 3International Institute of Molecular and Cell Biology in Warsaw

Tags

Primary AntibodySecondary AntibodyTransfected HeLa CellsMitochondrial DNA DistributionBSAPBSAlexa Fluor 488Alexa Fluor 555Anti-DNA AntibodiesMitochondrial Transcription Factor A (TFAM)Fluorescence Quantification

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Addition of Primary and Secondary Antibodies to the Transfected HeLa Cells to Monitor the mtDNA Distribution. J. Vis. Exp. (Pending Publication), e200043, doi:10.3791/200043 (2023).

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