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In vivo Imaging of Transgenic Leishmania Parasites in a Live Host
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09:53 min
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July 27, 2010
DOI:
1Interdisciplinary Immunology Program,University of Iowa, and the VA Medical Center, 2Department of Biochemistry,University of Iowa, and the VA Medical Center, 3Department of Internal Medicine,University of Iowa, 4Department of Molecular Microbiology,Washington University School of Medicine, 5Division of Dermatology,Harbor-UCLA Medical Center, Hanley-Hardison Research Center, 6Interdisciplinary Immunology Program,Iowa City VA Medical Center, 7Departments of Internal Medicine, Microbiology and Epidemiology,University of Iowa
Tags
In Vivo ImagingTransgenic Leishmania ParasitesLive HostLeishmania SpeciesVisceral LeishmaniasisCutaneous LeishmaniasisMouse ModelsParasite BurdensMicroscopyLimiting Dilution AssayQPCR AmplificationIn Vivo Imaging System (IVIS)Bioluminescent SignalLuciferase ExpressionIntradermal InjectionIntravenous InjectionLongitudinal StudiesInter-animal VariationParasite InoculaMultiplication Of ParasitesHigh SensitivityCutaneous Locations
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Thalhofer, C. J., Graff, J. W., Love-Homan, L., Hickerson, S. M., Craft, N., Beverley, S. M., Wilson, M. E. In vivo Imaging of Transgenic Leishmania Parasites in a Live Host. J. Vis. Exp. (41), e1980, doi:10.3791/1980 (2010).
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