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胚胎干细胞分化成少突胶质前体

JoVE Journal
Biologia

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JoVE Journal Biologia

Differentiation of Embryonic Stem Cells into Oligodendrocyte Precursors

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胚胎干细胞分化成少突胶质前体

DOI:

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08:11 min

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May 19, 2010

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Peng Jiang, Vimal Selvaraj, Wenbin Deng

¹Department of Cell Biology and Human Anatomy Institute for Pediatric Regenerative Medicine,School of Medicine, University of California, Davis

Capítulos

00:00Título
01:03Introduction
01:49Passaging Mouse Embryonic Stem Cells
03:18Generation Of Oligodendrocyte Precursor Cells From Mouse ES Cells
05:40Generation of Spiking Oligodendrocyte Precursor Cells
06:47Representative Results
07:31Conclusion

Summary

Tadução automática

我们描述了一种小分子为基础的小鼠胚胎干细胞分化成少突胶质前体细胞（OPCs的）协议。本协议产生高效率的30天的分化OLIG2 + NG2 + OPCs的。我们还描述了一种方法来生成可以触发动作电位的“尖峰”OPCs的。

Tags

Embryonic Stem Cells Oligodendrocyte Precursors Myelinating Cells Central Nervous System Regenerative Cell Therapy Demyelinating Diseases Transplantation Transcription Factor Olig2 Proteoglycan NG2 GFP-Olig2 Mouse Embryonic Stem Cell Reporter Line Differentiation Small Molecules Retinoic Acid Sonic Hedgehog Agonist Purmorphamine Electrophysiological Property NaV1.2 Subunit

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