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Magnetische Resonantie Spectroscopie van leven Drosophila melanogaster Met behulp van Magic Angle Spinning
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Neurociência
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JoVE Journal Neurociência
Magnetic Resonance Spectroscopy of live Drosophila melanogaster using Magic Angle Spinning
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Magnetische Resonantie Spectroscopie van leven Drosophila melanogaster Met behulp van Magic Angle Spinning

DOI:
10.3791/1710-v

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07:33 min

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April 15, 2010

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, , ,
1NMR Surgical Laboratory, Department of Surgery,Massachusetts General Hospital, Harvard Medical School, 2Shriners Burn Institute, 3Department of Radiology, Athinoula A. Martinos Center of Biomedical Imaging,Harvard Medical School, 4Molecular Surgery Laboratory, Department of Surgery,Massachusetts General Hospital, Harvard Medical School

Capítulos

  • 00:00Título
  • 00:43Introduction
  • 01:53Preparing Drosophila for HRMAS Measurements
  • 02:57HRMAS Rotor Preparation
  • 03:58HRMAS Data Acquisition
  • 05:27Data Processing/ Analysis
  • 06:05Representative Results/ Outcome
  • 06:50Conclusion

Summary

Tadução automática

Tadução automática

Deze techniek maakt het gebruik van hoge-resolutie magic angle spinning proton MR spectroscopie (HRMAS 1H-MRS) voor de moleculaire karakterisering van levende<em> Drosophila melanogaster</em> Met een conventionele 14,1 tesla spectrometer uitgerust met een HRMAS sonde.

Tags

Magnetic Resonance SpectroscopyLive Drosophila MelanogasterMagic Angle SpinningHigh-Resolution Magic Angle SpinningHRMAS Proton Magnetic Resonance Spectroscopy1H-MRSNon-destructive TechniqueSpectral Line-widthsHigh-resolution SpectraMetabolitesCellular ProcessesIn Vivo StudiesFruit Fly Drosophila MelanogasterGenetic OrganismBiological FunctionsSignaling PathwaysGenetics And PhysiologyIn Vivo HRMAS 1H-MRS Method14.1 TMR SpectrometerHRMAS ProbeMolecular CharacterizationDisease BiomarkersTherapeutic Development

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