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一种技术，同时可视化病毒特异性CD8 + T细胞和病毒感染的细胞原位</em
JoVE Journal
Biologia
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JoVE Journal Biologia
A Technique to Simultaneously Visualize Virus-Specific CD8+ T Cells and Virus-Infected Cells In situ
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一种技术，同时可视化病毒特异性CD8 + T细胞和病毒感染的细胞原位</em

DOI:
10.3791/1561-v

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20:47 min

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August 13, 2009

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, , ,
1Department of Microbiology, Medical School,University of Minnesota, 2Department of Veterinary and Biomedical Sciences,University of Minnesota

Capítulos

  • 00:07Título
  • 01:07Introduction
  • 02:18Preparation of Thick Fresh Tissue Sections
  • 05:22Staining the Thick Sections with Tetramers
  • 10:11Preparing Thin Sections
  • 12:17Acquiring Confocal Microscope Images
  • 17:56Representative Images of Simultaneous Visualization by ISTH of Immune Effectors and Targets in Tissue Sections
  • 19:50Conclusion

Summary

Tadução automática

Tadução automática

一个技术结合原位四聚体染色原位杂交（ISTH）使的病毒特定的CD8 + T细胞其病毒感染的目标空间邻近的可视化，制图和分析，并确定这些免疫效应和目标之间的定量关系得以实现感染的结果。

Tags

Virus-specific CD8+ T CellsVirus-infected CellsVisualizeIn SituImmune Effector CellsImmune DefensesInfectionSpatial ProximityQuantitative RelationshipsClearanceChronic Persistent InfectionsIn Situ Tetramer StainingIn Situ HybridizationViral-RNA+ CellsBattle Between Immune Defenses And InfectionISTH (in Situ Tetramer Staining And In Situ Hybridization)Mapping Of LocationsE:T RatiosTiming And Magnitude Of The Immune Response

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