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重新用四个转录因子的小鼠胚胎成纤维细胞诱导多能干细胞的产生，强力霉素诱导慢病毒转导系统
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November 13, 2009
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Stemgent阿霉素诱导小鼠TF慢病毒设置可以重新编程诱导多能干细胞（iPS细胞）的小鼠胚胎成纤维细胞（MEFs）。在这里，我们展示了鼠标重新编程转录因素Oct4的DOX诱导表达的协议，SOX2，KLF4和c - Myc基因产生的iPS殖民地，表达共同的MES多能性标志物。
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Induced Pluripotent Stem CellsReprogrammingTranscription FactorsDoxycycline Inducible Lentiviral Transduction SystemMouse Embryonic FibroblastsPluripotencyRetrovirus-mediated DeliveryOct4Sox2C-MycKlf4Doxycycline Inducible Lentiviral Delivery SystemAdult Mouse Somatic Cell TypesEmbryonic Stem CellsRegenerative MedicineTeratoma FormationDifferentiated CellsEthical DilemmaStemgent DOX Inducible Mouse TF Lentivirus Set
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Hamilton, B., Feng, Q., Ye, M., Welstead, G. G. Generation of Induced Pluripotent Stem Cells by Reprogramming Mouse Embryonic Fibroblasts with a Four Transcription Factor, Doxycycline Inducible Lentiviral Transduction System. J. Vis. Exp. (33), e1447, doi:10.3791/1447 (2009).
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