An Adjuvant Therapy in a Mouse Model with an Incompletely Resected Subcutaneous Tumor

Published: April 30, 2024

Abstract

Source: Rwandamuriye, F. X. et al., A Mouse Model of Incompletely Resected Soft Tissue Sarcoma for Testing (Neo)adjuvant Therapies. J. Vis. Exp. (2020)

This video illustrates testing of adjuvant or additional therapy in a mouse model with incompletely resected subcutaneous tumors. The partially tumor-resected mouse is treated with an antibody targeting the anti-cytotoxic protein of T regulatory cells, potentially reducing tumor regrowth and suggesting effective adjuvant therapy.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Inoculation of cells Preparation of cells and animals Ensure that the cell line is maintained in the recommended media. For example, maintain WEHI 164 cell line in Roswell Park Memorial Institute (RPMI) 1640 medium supplemented with 2 mM L-glutamine, 10% fetal bovine serum, 20 mM 4-(2-hydroxyethyl)-1-piperaz…

Representative Results

Figure 1: Representative images of the surgery setup. (A) A whole image of the surgery setup showing the surgical tools (listed in step 2.1) and the anesthetic machine. (B) A snapshot image of the surgical table showing all materials within easy reach. (C) A heating chamber and a heating pad for mouse recovery. <p class="jove_content" fo…

Declarações

The authors have nothing to disclose.

Materials

26 gauge 0.5 mL insulin syringe Becton Dickinson, Australia 326769 None
2-Mercaptoethanol Life Technologies Australia Pty Ltd 21985023 None
Anaestetic gas machine Darvall Vet, Australia SKU: 2848 None
Anti-CTLA-4 BioXcell, USA BE0164 None
Anti-PD-1 BioXcell, USA BP0273 None
Buprenorphine Hydrochloride Injection, 0.3mg/mL RB healthcare UK Limited, UK 55175 Prescription order
Chlorhexidine Surgical Scrub 4% Perigo Australia, Australia CHL01449F(scrub None
Fetal Bovine serum CellSera, Australia AU-FBS-PG None
Forceps Fine 10.5 cm Surgical house, Western Australia CC74110 None
Forceps Fine 12 cm Serrated Surgical house, Western Australia CC74212 None
Forceps Halsted 14 cm Surgical house, Western Australia CD01114 None
Heating chamber Datesand Ltd, UK Mini-Thermacage None
HEPES (1M) Life Technologies Australia Pty Ltd 15630080 None
Isoflurane Henry Schein Animal Health, Australia SKU: 29405 Prescription order
Lubricating Eye Ointment Alcon n/a None
Penicillin/streptomycin 1000X Life Technologies Australia Pty Ltd 15140122 None
Phosphate Buffered Solution 10x Life Technologies Australia Pty Ltd 70013-032 None
Reflex 7mm Clips Able scientific, Australia AS59038 None
Reflex 7mm Wound Clip Applicator Able scientific, Australia AS59036 None
Reflex Wound Clip Remover Able scientific, Australia AS59037 None
Rodent Qube Anesthesia Breathing Circuit Darvall Vet, Australia #7885 None
Roswell Park Memorial Institute (RPMI) 1640 Medium + L-glutamine Life Technologies Australia Pty Ltd 21870092 None
Scissors Iris STR 11 cm Surgical house, Western Australia KF3211 None
Scissors Iris STR 9 cm Surgical house, Western Australia JH4209 None
Small Induction Chamber Darvall Vet, Australia SKU: 9630 None
TrypLE express 1x Life Technologies Australia Pty Ltd 12604-021 None

Tags

Play Video

Citar este artigo
An Adjuvant Therapy in a Mouse Model with an Incompletely Resected Subcutaneous Tumor. J. Vis. Exp. (Pending Publication), e22228, doi: (2024).

View Video