An Adjuvant Therapy in a Mouse Model with an Incompletely Resected Subcutaneous Tumor
Published: April 30, 2024
Abstract
Source: Rwandamuriye, F. X. et al., A Mouse Model of Incompletely Resected Soft Tissue Sarcoma for Testing (Neo)adjuvant Therapies. J. Vis. Exp. (2020)
This video illustrates testing of adjuvant or additional therapy in a mouse model with incompletely resected subcutaneous tumors. The partially tumor-resected mouse is treated with an antibody targeting the anti-cytotoxic protein of T regulatory cells, potentially reducing tumor regrowth and suggesting effective adjuvant therapy.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Inoculation of cells Preparation of cells and animals Ensure that the cell line is maintained in the recommended media. For example, maintain WEHI 164 cell line in Roswell Park Memorial Institute (RPMI) 1640 medium supplemented with 2 mM L-glutamine, 10% fetal bovine serum, 20 mM 4-(2-hydroxyethyl)-1-piperaz…
Representative Results
Figure 1: Representative images of the surgery setup. (A) A whole image of the surgery setup showing the surgical tools (listed in step 2.1) and the anesthetic machine. (B) A snapshot image of the surgical table showing all materials within easy reach. (C) A heating chamber and a heating pad for mouse recovery. <p class="jove_content" fo…
Declarações
The authors have nothing to disclose.
Materials
| 26 gauge 0.5 mL insulin syringe | Becton Dickinson, Australia | 326769 | None |
| 2-Mercaptoethanol | Life Technologies Australia Pty Ltd | 21985023 | None |
| Anaestetic gas machine | Darvall Vet, Australia | SKU: 2848 | None |
| Anti-CTLA-4 | BioXcell, USA | BE0164 | None |
| Anti-PD-1 | BioXcell, USA | BP0273 | None |
| Buprenorphine Hydrochloride Injection, 0.3mg/mL | RB healthcare UK Limited, UK | 55175 | Prescription order |
| Chlorhexidine Surgical Scrub 4% | Perigo Australia, Australia | CHL01449F(scrub | None |
| Fetal Bovine serum | CellSera, Australia | AU-FBS-PG | None |
| Forceps Fine 10.5 cm | Surgical house, Western Australia | CC74110 | None |
| Forceps Fine 12 cm Serrated | Surgical house, Western Australia | CC74212 | None |
| Forceps Halsted 14 cm | Surgical house, Western Australia | CD01114 | None |
| Heating chamber | Datesand Ltd, UK | Mini-Thermacage | None |
| HEPES (1M) | Life Technologies Australia Pty Ltd | 15630080 | None |
| Isoflurane | Henry Schein Animal Health, Australia | SKU: 29405 | Prescription order |
| Lubricating Eye Ointment | Alcon | n/a | None |
| Penicillin/streptomycin 1000X | Life Technologies Australia Pty Ltd | 15140122 | None |
| Phosphate Buffered Solution 10x | Life Technologies Australia Pty Ltd | 70013-032 | None |
| Reflex 7mm Clips | Able scientific, Australia | AS59038 | None |
| Reflex 7mm Wound Clip Applicator | Able scientific, Australia | AS59036 | None |
| Reflex Wound Clip Remover | Able scientific, Australia | AS59037 | None |
| Rodent Qube Anesthesia Breathing Circuit | Darvall Vet, Australia | #7885 | None |
| Roswell Park Memorial Institute (RPMI) 1640 Medium + L-glutamine | Life Technologies Australia Pty Ltd | 21870092 | None |
| Scissors Iris STR 11 cm | Surgical house, Western Australia | KF3211 | None |
| Scissors Iris STR 9 cm | Surgical house, Western Australia | JH4209 | None |
| Small Induction Chamber | Darvall Vet, Australia | SKU: 9630 | None |
| TrypLE express 1x | Life Technologies Australia Pty Ltd | 12604-021 | None |
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Citar este artigo
An Adjuvant Therapy in a Mouse Model with an Incompletely Resected Subcutaneous Tumor. J. Vis. Exp. (Pending Publication), e22228, doi: (2024).