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Detectie van polyfunctionele T-cellen bij kinderen die zijn gevaccineerd met japans encefalitisvaccin via de flowcytometrietechniek
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Detectie van polyfunctionele T-cellen bij kinderen die zijn gevaccineerd met japans encefalitisvaccin via de flowcytometrietechniek
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September 23, 2022
DOI:
1Beijing Key Laboratory of Pediatric Respiratory Infectious Diseases, Key Laboratory of Major Diseases in Children, Ministry of Education, National Clinical Research Center for Respiratory Diseases, Laboratory of Infection and Virology, Beijing Pediatric Research Institute, Beijing Children’s Hospital,Capital Medical University, National Center for Children’s Health, 2Research Unit of Critical Infection in Children,2019RU016, Chinese Academy of Medical Sciences, 3Department of Pediatric Rehabilitation, Beijing Boai Hospital, School of Rehabilitation Medicine,Capital Medical University, China Rehabilitation Research Center, 4Center of Excellence (Beijing),Becton Dickinson Medical Devices (Shanghai) Co Ltd
Summary
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Het huidige protocol combineert ex vivo stimulatie en flowcytometrie om polyfunctionele T-cel (TPF) profielen te analyseren in perifere mononucleaire bloedcellen (PBMC's) binnen Japanse encefalitisvirus (JEV) -gevaccineerde kinderen. De detectiemethode en het flowcytometriekleurenschema van JEV-specifieke TPF's werden getest om een referentie te bieden voor vergelijkbare studies.
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Zhang, L., Zhang, M., Liu, M., Ai, J., Tian, J., Ge, H., Wang, R., Xie, Z. Detection of Polyfunctional T Cells in Children Vaccinated with Japanese Encephalitis Vaccine via the Flow Cytometry Technique. J. Vis. Exp. (187), e64671, doi:10.3791/64671 (2022).
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