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使用Leginon进行载物台倾斜的单颗粒冷冻电镜数据收集
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04:52 min
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July 01, 2022
DOI:
1Laboratory of Genetics,The Salk Institute for Biological Studies, 2Jack H. Skirball Center for Chemical Biology and Proteomics,The Salk Institute for Biological Studies, 3Department of Chemistry and Biochemistry,University of California San Diego, 4Graduate School of Biological Sciences, Section of Molecular Biology,University of California San Diego, 5Department of Integrative Structural and Computational Biology,The Scripps Research Institute
Summary
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本协议描述了冷冻电镜实验中倾斜单颗粒数据收集的通用且易于实施的方案。对于由于粘附空气-水界面而遭受优先方向偏差的样品，该程序对于获得高质量的EM图特别有用。
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Cite this Article
Aiyer, S., Strutzenberg, T. S., Bowman, M. E., Noel, J. P., Lyumkis, D. Single-Particle Cryo-EM Data Collection with Stage Tilt using Leginon. J. Vis. Exp. (185), e64136, doi:10.3791/64136 (2022).
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