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通过直接生物测定法鉴定海葵粗提取物中的溶血和磷脂酶活性
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Identification of Hemolytic and Phospholipase Activity in Crude Extracts from Sea Anemones by Straightforward Bioassays
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通过直接生物测定法鉴定海葵粗提取物中的溶血和磷脂酶活性

2,414 Views

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12:12 min

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March 29, 2022

DOI:

10.3791/63630-v

DOI:
10.3791/63630-v

•

12:12 min
March 29, 2022

•

2 Views
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1Instituto de Biotecnología,Universidad Nacional Autónoma de México, 2Departamento de Micro y Nanotecnologías,Instituto de Ciencias Aplicadas y Tecnología, Universidad Nacional Autónoma de México

Summary

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在这里，我们描述了一种从海葵中获得粗毒血清提取物并检测其溶血和磷脂酶活性的方案。

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Keyword Extraction:Hemolytic ActivityPhospholipase ActivitySea Anemone ToxinBioassaysCrude ExtractProteinHemolysisPhospholysisErythrocyteTemperatureLyophilizationCell LysisNematocyst DischargeCentrifugationBradford Colorimetric Assay

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Cite this Article

Ramírez-Carreto, S., Salazar-García, S. I., Macías Martínez, G., Rodríguez-Almazán, C. Identification of Hemolytic and Phospholipase Activity in Crude Extracts from Sea Anemones by Straightforward Bioassays. J. Vis. Exp. (181), e63630, doi:10.3791/63630 (2022).

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