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Cryo-section Dissection of the Adult Subependymal Zone for Accurate and Deep Quantitative Proteome Analysis
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신경과학
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Cryo-section Dissection of the Adult Subependymal Zone for Accurate and Deep Quantitative Proteome Analysis

Cryo-section Dissection of the Adult Subependymal Zone for Accurate and Deep Quantitative Proteome Analysis

3,101 Views

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06:24 min

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October 07, 2021

DOI:

10.3791/63047-v

DOI:
10.3791/63047-v

•

06:24 min
October 07, 2021

•

2 Views
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1Division of Physiological Genomics, Biomedical Center,Ludwig Maximilian University of Munich, 2Institute for Stem Cell Research,Helmholtz Zentrum München, 3SYNERGY, Excellence Cluster Systems Neurology,University of Munich, 4Department of Clinical Neuroscience,Karolinska Institutet

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Cryo-section-dissection allows fresh, frozen preparation of the largest neurogenic niche in the murine brain for deep quantitative proteome analysis. The method is precise, efficient, and causes minimal tissue perturbation. Therefore, it is ideally suited for studying the molecular microenvironment of this niche, as well as other organs, regions, and species.

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Cryo-sectionSubependymal ZoneQuantitative Proteome AnalysisVentricular Neurogenic NicheDissection MethodMass SpectrometryMouse BrainLateral VentriclesCortexCorpus CallosumChoroid PlexusCryostat

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Friess, C., Götz, M., Kjell, J. Cryo-section Dissection of the Adult Subependymal Zone for Accurate and Deep Quantitative Proteome Analysis. J. Vis. Exp. (176), e63047, doi:10.3791/63047 (2021).

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