Journal
/
면역학 및 감염병학
/
Selective Cleaning of Wild Caenorhabditis Nematodes to Enrich for Intestinal Microbiome Bacteria
JoVE 신문
면역학 및 감염병학
This content is Free Access.
JoVE 신문 면역학 및 감염병학
Selective Cleaning of Wild Caenorhabditis Nematodes to Enrich for Intestinal Microbiome Bacteria

Selective Cleaning of Wild Caenorhabditis Nematodes to Enrich for Intestinal Microbiome Bacteria

DOI:
10.3791/62937-v

•

09:47 min

•

August 13, 2021

•

, , ,
1Department of Biology,San Diego State University, 2Institut de Biologie de l’École Normale Supérieure

Chapters

  • 00:04Introduction
  • 00:34Inducing Dauer Formation for Wild Nematodes on Nematode Growth Medium (NGM) Plates
  • 01:16Washing and Disinfecting the Nematodes
  • 03:12Removing the Antibiotic and SDS Solution
  • 04:08Propagate a Clean Nematode Strain
  • 05:27Intestinal Dissection and PCR Identification of Microbial Species
  • 08:17Results: The Host-microbe Interactions in the Context of the Intestines of Caenorhabditis tropicalis Strain JU1848
  • 09:16Conclusion

Summary

자동 번역

자동 번역

Wild Caenorhabditis nematodes are associated with many microbes, often in the gut lumen or infecting the intestine. This protocol details a method to enrich unculturable microbes colonizing the intestine, taking advantage of the resistance of the dauer cuticle.

Tags

CaenorhabditisNematodesMicrobiomeEnrichmentIntestinal MicrobiomeUnculturable MicrobesOP51 Escherichia ColiDauer StageM9 Minimal Salts MediaTriton X-100AntibioticSDS Solution

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

Application of a Mouse Ligated Peyer’s Patch Intestinal Loop Assay to Evaluate Bacterial Uptake by M cells

A Protocol to Infect Caenorhabditis elegans with Salmonella typhimurium

Biomimetic Materials to Characterize Bacteria-host Interactions

Selective Harvesting of Marginating-pulmonary Leukocytes

Selective Harvesting of Marginating-hepatic Leukocytes

Development of an Antigen-driven Colitis Model to Study Presentation of Antigens by Antigen Presenting Cells to T Cells

Immunofluorescence Analysis of Stress Granule Formation After Bacterial Challenge of Mammalian Cells

Assaying for Inorganic Polyphosphate in Bacteria

Visualization of Gut Microbiota-host Interactions via Fluorescence In Situ Hybridization, Lectin Staining, and Imaging

Chemical Cartography Approaches to Study Trypanosomatid Infection

Read Article