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在麻醉和清醒行为的非人灵长类动物大脑中注射用于光遗传学的AAV载体

JoVE 신문
신경과학

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JoVE 신문 신경과학

Injections of AAV Vectors for Optogenetics in Anesthetized and Awake Behaving Non-Human Primate Brain

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在麻醉和清醒行为的非人灵长类动物大脑中注射用于光遗传学的AAV载体

3,177 Views

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09:42 min

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August 04, 2021

DOI:

10.3791/62546-v

DOI:

10.3791/62546-v

•

09:42 min

August 04, 2021

•

9 Views

Yoshiko Kojima^1,2, Jonathan T. Ting^2,3,4, Robijanto Soetedjo^2,4, Shane D. Gibson^2,4, Gregory D. Horwitz^2,4

¹Dept. of Otolaryngology – Head and Neck Surgery,University of Washington, ²Washington National Primate Research Center,University of Washington, ³Allen Institute for Brain Science, ⁴Dept. of Physiology & Biophysics,University of Washington

Summary

正如目前所实施的那样，非人灵长类动物的光遗传学需要将病毒载体注射到大脑中。最佳注射方法应该是可靠的，并且对于许多应用，能够靶向任意深度的单个位点，这些位点在尸检组织学中很容易明确地识别出来。提出了一种具有这些特性的注射方法。

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